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History of Science and Engineering Annotation << Back
P L A S M I D S
C O N T R O L L I N G
BIODEGRADATION
O F A R O M A T I C
HYDROCARBONS |
I.A. Kosheleva S.L. Sokolov O.I. Sazonova T. Yu.Izmalkova A.M. Boronin
Over 100 strains degrading polycyclic aromatic hydrocarbons were isolated and studied. A comparative analysis of the organization of
the genetic systems of naphthalene and phenanthrene biodegradation in fl uorescent pseudomonads, including nah1- and nah2- operons, was
performed. The ability of Pseudomonas strains to utilize naphthalene in most cases controlled by large conjugative plasmids. Most often,
plasmid genes for naphthalene degradation are organized in two operons: nah1 (oxidation of naphthalene to salicylate) and nah2 (oxidation
of salicylate to catechol with further meta -pathway cleavage to intermediates of the TCA cycle). In some cases naphthalene degradation is
controlled by plasmid and chromosomal genes, or the genes located on two different plasmids. In strains BS202, BS3701, BS3790, there are
three non-homologous gene salicylate hydroxylase (nahG, nahG1 and nahU). Degradation of salicylate in these strains is carried out with the
participation of the nahU gene located apart. The plasmid incompatibility group P-9 can be divided into at least fi ve subgroups on the basis
of their basic replicons structural organization. The studied replicons of IncP-7 plasmids possess a highly conservative organization and are
divided into four subgroups. Plasmids belonging to the IncP-9 group are usually presented in the soil independently from contamination. The
pool of IncP-7 plasmids is increased in response to the soil contamination.
Keywords: plasmids; biodegradation; aromatic hydrocarbons; incompatibility groups; catabolic operons.
Contacts: E-mail: kosheleva@ibpm.pushchino.ru
Pp. 47-58. |
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