History of Science and Engineering Annotation << Back RECENT METHODS FOR CREATING MICROBIAL BIOSENSORS AND BIOFUEL ELEMCELLS A.N. Reshetilov This article is a presentation of the results obtained through scientific studies on the design of electrochemical biosensor analyzers and biofuel cells. This work has been done over the past five years at the Institute of Biochemistry and Physiology of Microorganisms, RAS in the Laboratory of biosensors. For this purpose, a particular focus was placed on the use of microbial cells; in some cases, the experiments were carried out using enzymes. The main attention was paid to the study of new properties of microbial biosensors (mBSs) and microbial biofuel cells (mBFCs), which could be constructed in combination with nanomaterials such as thermoexpanded graphite (TEG), single- and multi-wall carbon nanotubes. The main results have been obtained while fabricating composite electrolytes which contain the enzyme immobilized into multi-layer polyelectrolyte capsules. At the same time, the properties of conventional mBSs developed for detection of alcohols, carbohydrates xenobiotics were explored. A mBFE-based system for charge accumulation in the converter has been constructed. This system was used to raise the initial potential of the fuel element from 0,3 V to 3,2 V. Our estimated data showed how the grass frog Rana temporaria can be used as a source of electric energy generation after glucose oxidation in the frog body. The possibility of applying this system in practice is discussed. Keywords: microbial cells, biosensors, biofuel cells, anode nanomaterial, charge accumulation in the converter, frog body as an energy source. DOI: 10.25791/intstg.06.2020.1187 Pp. 15-23.