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Biotechnological and Molecular-Genetic Methods for Preservation and Reproduction Useful and Rare Species Of Plants
Molkanova O.I., Vasil’eva O.G., Mamaeva N.A., Vetchinkina E.M., Konovalova T.Y.

The application of isolated plant tissue and organ culture is coming more and more actual along with traditional plant ex situ conservation methods. The highly efficient clonal micropropagation technologies have been improved for more than 1 000 plant species related to 32 families and 85 genera. Most of the collection including more than 1 000 angiosperm genotypes is stored under conditions providing minimum growth processes (3–5 °C). During the creation of gene banks, plant species representativeness and genetic stability preservation is given a high priority. Evaluation of the relative genetic distance between microclones and known taxa is proposed as a method to verify in vitro germplasm collections. Key words: genetic diversity; identification; bank of aseptic ciltures; organogenesis; conservation; in vitro. E-mail: molkanova@mail.ru; yarilko@inbox.ru; konovtat@mail.ru

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